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. Author manuscript; available in PMC: 2021 Mar 18.
Published in final edited form as: J Immunol. 2020 Jan 13;204(5):1263–1273. doi: 10.4049/jimmunol.1901066

FIGURE 4.

FIGURE 4.

B. licheniformis PGA induces DC maturation. Human iDCs were stimulated with 100 ng/ml LPS, 500 μg/ml B. licheniformis PGA, or 500 μg/ml B. subtilis PGA or were maintained in medium alone. Two days later, the cells were assessed for expression of MHC I, MHC II, CD83, and CCR7 by flow cytometry. Six experiments were run using cells from six donors. Histograms and mean fluorescence intensities (MFI) shown are from a single representative experiment. (A) The filled histograms represent the isotype control and the outlined histograms represent the marker staining for the control iDCs. (B–E) The filled histograms represent the marker staining for the control iDCs. The outlined histograms represent the marker staining for the cells treated with LPS (B), B. licheniformis PGA (C), B. subtilis PGA (D), or B. anthracis PGA (E). (F and G) The cells were run in CCR7-dependent chemotactic activity assays using CCL19 and CCL21 as chemoattractants. Mean data are presented as chemotactic indices ± SEM (n = 4 experiments). Statistical significance was determined by t tests comparing the mean chemotactic index for each of the treatments to the iDC control. ***p < 0.001, ****p < 0.0001. Bl, B. licheniformis; Bs, B. subtilis.