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Relative iNOS mRNA expression normalized to murine HPRT at different time points in A. phagocytophilum infected WT, DAP12-/-and FcRγ-/- Hoxb8 neutrophils. Results were normalized to the 0 h value of uninfected WT cells using the ΔΔCt-method. Mean and SD from 5 independent experiments are shown. Differences between gene-deficient Hoxb8 neutrophils and WT control cells at each time point were analyzed using the two-tailed Mann–Whitney test.
