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. 2021 Mar 4;11:627630. doi: 10.3389/fcimb.2021.627630

Figure 3.

Figure 3

Relative iNOS mRNA expression normalized to murine HPRT at different time points in uninfected, A. phagocytophilum infected and LPS-stimulated WT (A), TLR-5-fold-/- (B), MyD88-/- (C) and MyD88-/- TRIF-/- (D) Hoxb8 neutrophils. Infected (E) and LPS-stimulated (F) WT and gene-deficient Hoxb8 neutrophils were compared side by side in panels (E) and (F). Results were normalized to the 0 h value of uninfected WT cells using the ΔΔCt-method. Mean and SD from five independent experiments are shown. Differences between experimental groups were analyzed using the two-tailed Mann–Whitney test. The following groups were compared: WT, TLR-5-fold-/-, MyD88-/-, and MyD88-/- TRIF-/- A. phagocytophilum infected or LPS-stimulated Hoxb8 neutrophils to the respective uninfected control cells at each time point (A–D), A. phagocytophilum infected or LPS-stimulated TLR-5-fold-/-, MyD88-/-, and MyD88-/- TRIF-/- Hoxb8 neutrophils to A. phagocytophilum infected or LPS-stimulated WT cells (E, F). *p < 0.05, **p < 0.01.