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. 2021 Mar 4;11:627630. doi: 10.3389/fcimb.2021.627630

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Copyright © 2021 Müller, Westheider, Birkner, Seelig, Kirschnek, Bogdan and von Loewenich

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative iNOS mRNA expression normalized to murine HPRT in WT, TLR7^-/-, TLR9^-/- and TRIF^-/- Hoxb8 neutrophils at different time points after infection with A. phagocytophilum. Results were normalized to the 0 h value of uninfected WT cells using the ΔΔC_t-method. Mean and SD from five independent experiments are shown. Differences between experimental groups were analyzed using the two-tailed Mann–Whitney test. The following groups were compared: the respective A. phagocytophilum infected gene-deficient Hoxb8 neutrophils to WT control cells.