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. Author manuscript; available in PMC: 2021 Oct 27.
Published in final edited form as: ACS Nano. 2020 Sep 25;14(10):12732–12748. doi: 10.1021/acsnano.0c03064

Figure 2: Comparative analysis of keratinocyte-derived exosome with exosome isolated from cell culture conditioned media.

Figure 2:

(A) Schematic diagram showing exosome isolation process from murine keratinocyte (Kera308) cultured media with and without transfection with keratin 14 promoter-driven recombinant plasmids encoding CD9, CD63 or CD81 with “in frame” GFP reporter. (B) The particle size distribution of exosomes and eluent (membrane particles and apoptotic bodies) from murine keratinocyte cultured media. (n=6) (C) Representative Transmission Electron Microscopy (TEM) images of exosomes isolated using GFP beads and CD-beads. Scale, 200 nm (D) Flow cytometric analysis of murine keratinocyte-derived exosome on CD magnetic beads (CD63, CD9 and CD81) and GFP-beads showing binding of TSG-101 PE antibody. (E-F) Binding of TSG-101 PE with the murine keratinocyte-derived exosome was further tested by autocorrelation curves as determined by fluorescence correlation spectroscopy (FCS) (E) and time-resolved fluorescence anisotropy (F) (n=20). All data were shown as mean ± SEM. Data in F were analyzed by one-way ANOVA with the post-hoc Bonferroni’s multiple comparison test.