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. Author manuscript; available in PMC: 2021 Oct 27.
Published in final edited form as: ACS Nano. 2020 Sep 25;14(10):12732–12748. doi: 10.1021/acsnano.0c03064

Figure 3. Characterization of keratinocyte-derived exosome from murine skin and wound-edge tissue.

Figure 3.

(A) Murine dorsal skin was transfected with keratin 14 promoter-driven recombinant plasmids encoding CD9, CD63 or CD81 with “in frame” GFP reporter via TNT. The keratinocyte-derived exosomes were isolated from skin and day 5 wound-edge tissue using GFP magnetic trap and quantified using NTA. Data shown as mean ± SEM and were analyzed by two-tailed unpaired Student’s t-test. (n=8) (B) High-resolution automated electrophoresis of RNA isolated from skin and wound-edge Exoκ-GFP. RNA in wound-edge Exoκ-GFP was significantly abundant compared to that of skin Exoκ-GFP.