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. Author manuscript; available in PMC: 2021 Oct 27.
Published in final edited form as: ACS Nano. 2020 Sep 25;14(10):12732–12748. doi: 10.1021/acsnano.0c03064

Figure 7: TLNPk/si-hnRNPA2B1 inhibits packaging of miRNA within the exosome in keratinocyte.

Figure 7:

(A) Schematic diagram showing experimental design to test the efficacy of TLNPκ/si-hnRNPA2B1 in inhibiting miRNA packaging within the exosome in keratinocyte. (B) Western blot analysis of hnRNPA2B1 in human keratinocytes 72 h after treatment with TLNPκ encapsulating either si-control or si-hnRNPA2B1. Quantification of hnRNPA2B1 expression from immunoblots. (n=8,7) (C) The exosomes isolated from keratinocyte conditioned media 48h after transfection and Nanoparticle Tracking Analysis was done. The exosome concentration in the conditioned media 48 h after treatment with TLNPκ encapsulating either si-control or si-hnRNPA2B1were plotted graphically. (n=12) (D) High-resolution automated electrophoresis of RNA isolated from exosomes in the conditioned media 48 h after treatment with TLNPκ encapsulating either si-control or si-hnRNPA2B1were plotted graphically. (E) The RNA concentration per 109 exosomes in the conditioned media 48 h after treatment with TLNPκ encapsulating either si-control or si-hnRNPA2B1were plotted graphically. (n=12) (F) The abundance of miR-21–5p in exosome isolated from the conditioned media 48 h after treatment with TLNPκ encapsulating either si-control or si-hnRNPA2B1were plotted graphically. (n=12). Data in B, C, E and F were shown as mean ± SEM and were analyzed by two-tailed unpaired Student’s t-test.