Figure 3. Heterologous expression of recombinant protein Hisx6MBP-AgCecropB and E. coli BL21 (DE3) cell lysis. (A) 12% SDS-PAGE of Hisx6MBP-AgCecropB expression using IPTG (0.5 mM) to induce expression, at 28 °C. P: molecular mass standard; A.I.: aliquot before induction; 1h, 2h, 3h, 4h, ON: aliquots after 1, 2, 3, 4 and 16 hours after induction. Arrow points to Hisx6MBP-AgCecropB expected molecular mass (≅ 46 kDa). (B) E. coli BL21 (DE3) cell lysis after 3 hours of induction. P: molecular mass standard; A.I.: aliquot before induction; 3h: 3 hours after induction; Sob: soluble fraction after lysis process; Pe: precipitate fraction after lysis process. Arrow points to Hisx6MBP-AgCecropB expected molecular mass (48.5 kDa).