Table VI.
Bisphenol assessment in cell culture and ART media (in ng/l).
| Bisphenols (LOQ in ng/l) |
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample number | Product type | Name | Reference number | Batch number | Use | BPA (10) | BPS (2) | BPE (10) | BPF (5) | BPAF (5) | BPAP (10) | BPB (5) | BPC (15) | BPP (10) | BPZ (10) |
| 18 | Cell culture medium | McCoy's 5A Medium | M8403 | SLCB0586 | RL | NQ | 74 | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ |
| 19 | SLCB7211 | NQ | 395 | NQ | NQ | 5 | NQ | NQ | NQ | NQ | NQ | ||||
| 20 | Oocyte retrieval, holding and washing media | Synvitroflush | 15840125A | 20250050 | ART | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ |
| 21 | 20150031 | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | ||||
| 22 | Flushing medium | 10840060A | 20280036 | ART | NQ | 456 ± 3 | 12 ± 0.3 | 15 ± 0.6 | NQ | NQ | NQ | NQ | NQ | NQ | |
| 23 | 20040044 | 5 ± 0.4 | 436 ± 3 | 28 ± 0.3 | NQ | NQ | NQ | NQ | NQ | NQ | NQ | ||||
| 24 | BO-WASH | 61008 | WASH1704 | BEP | NQ | 35 ± 0.2 | NQ | 6 ± 0.1 | NQ | NQ | NQ | NQ | NQ | NQ | |
| 25 | Medium 199 with Hepes | M7528 | RNBG6724 | BEP | 28 | 308 | 15 | NQ | NQ | 14 | NQ | NQ | NQ | NQ | |
| 26 | In vitro maturation media | BO-IVM | 61002 | IVM1701N | BEP | 28 ± 1.4 | 53 ± 0.9 | NQ | 17 ± 1.4 | NQ | 16 ± 0.5 | NQ | NQ | NQ | NQ |
| 27 | BO-HEPES-IVM | 61009 | IVMH1602N | BEP | 155 ± 2 | 23 ± 0.6 | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | |
| 28 | Sperm preparation and IVF media | BO-IVF | 61003 | IVF1702N | BEP | 62 ± 1 | 112 ± 2 | 64 ± 1 | NQ | NQ | 38 ± 1.7 | NQ | NQ | NQ | NQ |
| 29 | Gradient 40/80 | 84022060 A | 20110060 | ART | NQ | 67 ± 1 | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | |
| 30 | 20180042 | NQ | 21 ± 1 | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | ||||
| 31 | Sequential Fert | 83010060A | 20190042 | ART | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | |
| 32 | 20220035 | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | ||||
| 33 | Universal IVF medium | 10310060 A | 20270017 | ART | NQ | 750 ± 24 | 18 ± 0.6 | 20 ± 1.5 | 10 ± 0.4 | NQ | NQ | NQ | NQ | NQ | |
| 34 | In vitro development media | SAGE 1-step | 67010010A | 19370063 | ART | NQ | 283 | 16 | NQ | NQ | NQ | NQ | NQ | NQ | NQ |
| 35 | 19290061 | 12 | 394 | 18 | NQ | 11 | NQ | NQ | NQ | NQ | NQ | ||||
| 36 | 20270046 | NQ | 337 ± 2 | NQ | 9 ± 1.3 | NQ | NQ | NQ | NQ | NQ | NQ | ||||
| 37 | Sequential Cleav | 83040010A | 20320064 | ART | 18 ± 0.7 | 179 ± 4 | 7 ± 1.3 | 8 ± 1.2 | 7 ± 0.5 | NQ | NQ | NQ | NQ | NQ | |
| 38 | Sequential Blast | 83060010A | 20330063 | ART | 4 ± 0.6 | 187 ± 20 | NQ | 3 ± 0.3 | 5 ± 0.1 | NQ | NQ | NQ | NQ | NQ | |
| 39 | Global | LGGG-020 | LGGG-200824U | ART | NQ | 10 ± 0.1 | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | |
| 40 | BO-IVC | 61001 | IVC1703N | BEP | 34 ± 3.8 | 322 ± 4 | 119 ± 6 | 25 ± 2.6 | NQ | 16 ± 0.6 | NQ | NQ | NQ | NQ | |
| 41 | Medium 199—100 ml bottle | M4530 | RNBH8521 | BEP | NQ | 278 | 16 | 5 | NQ | NQ | NQ | NQ | NQ | NQ | |
| 42 | RNBG5443 | NQ | 1188 ± 23 | 26 ± 4.6 | 18 ± 2.3 | NQ | NQ | NQ | NQ | NQ | NQ | ||||
| 43 | RNBG5443 | NQ | 1693 | 33 | 16 | NQ | NQ | NQ | NQ | NQ | NQ | ||||
| 44 | RNBG5443 | NQ | 1220 | 21 | 18 | NQ | NQ | NQ | NQ | NQ | NQ | ||||
| 45 | RNBH6994 | NQ | 233 | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ | ||||
| 46 | Embryo washing and handling media | Global with HEPES | LGGH-050 | LGGH-200820C | ART | NQ | 5 ± 0.1 | NQ | NQ | NQ | NQ | NQ | NQ | NQ | NQ |
NQ, not quantifiable; LOQ, mentioned below each bisphenols between brackets; each sample number corresponds to a separate vial; bold text: significant difference with the controls P < 0.0001, non-parametric one-way ANOVA with the Tukey post hoc test were performed; sample number 18, 19, 25, 34, 35, 41, 43, 44 and 45 were not analysed in triplicate.