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. Author manuscript; available in PMC: 2021 Mar 18.
Published in final edited form as: Am J Surg. 2013 Feb 19;205(4):387–396. doi: 10.1016/j.amjsurg.2013.01.008

Figure 1.

Figure 1

Triptolide treatment in neuroblastoma cells results in reduced cell viability with alternative mechanisms of cell death. Triptolide treatment in both neuroblastoma cell lines resulted in concentration- and time-dependent responses in vitro at minimal concentrations. (A) Cell death occurred in less than 72 hours at a 100 nmol/L concentration in both cell lines, with 45% cell death in IMR-32 cells and 50% cell death in SH-SY5Y cells. (B) A concentration- and time-dependent increase in caspase-3 activity was demonstrated with triptolide treatment in the IMR-32 cell line, which is consistent with cell death by apoptosis. However, this effect was not seen in the SH-SY5Y cell line, suggesting a method of cell death other than apoptosis. Data expressed as the mean ± SEM of 3 independent experiments. *P < .05 (t test) as compared with controls.