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. 2021 Mar 4;12:603649. doi: 10.3389/fimmu.2021.603649

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Copyright © 2021 Mountford, Effenberger, Noll-Puchta, Griessmair, Ringleb, Haas, Denk, Reiter, Mayr, Dinarello, Tilg and Bufler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-37 overexpression reduces the pro-inflammatory response of LX2 stellate cells. LX2 cells were transfected with two different IL-37 expressing cmRNA or vehicle or exposed to rhIL-37 protein. Cells were stimulated with IL-1β (1 ng/ml) 24 h after transfection or treatment with rhIL-37. (A) IL-37 receptor expression. SIGIRR and IL-18Rα mRNA from LX2 and human PBMC was detected by RT-PCR. (B) Lysates of LX2 cells transfected with IL-37 cmRNA1, cmRNA2 or control mRNA were analyzed for IL-37 protein expression by western blot. (C) IL-6 in supernatants of IL-37 mRNA-transfected LX2 cells after IL-1β-stimulation. (D) mRNA levels by qPCR 6 h after IL-1β-stimulation and are expressed relative to control mRNA transfection. (E) IL-1β-stimulated LX2 cells treated with rhIL-37. IL-6 in cell supernatants was measured after 24 h stimulation. n = 3, *p < 0.05.