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. 2021 Mar 4;12:603649. doi: 10.3389/fimmu.2021.603649

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Copyright © 2021 Mountford, Effenberger, Noll-Puchta, Griessmair, Ringleb, Haas, Denk, Reiter, Mayr, Dinarello, Tilg and Bufler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recombinant IL-37 protein does not modulate the pro-inflammatory response of HSC. (A) Recombinant IL-37 (1 μg/ml) was added every second day along with fresh media to cultured Wt HSC. Supernatants were collected on day 5, 7, and 9 during myofibroblast differentiation and tested for spontaneous IL-6 release. (B) Freshly isolated mHSC from Wt mice were cultured with 1 μg/ml rhIL-37 overnight and subsequently stimulated with LPS (100 ng/ml). mRNA levels were measured by qPCR 6 h after stimulation. Fold changes of mRNA expression were calculated using the ΔΔCt-method normalized to Rpl13a gene expression. Open boxes/bars: PBS, closed boxes/bars: rhIL-37. N = 3.