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. 2021 Mar 10;11:5546. doi: 10.1038/s41598-021-85129-1

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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NSC-6 antibody immunolabels different adult mouse brain regions. (A) NSC-6 immunopositive glia-like cells in the corpus callosum (cc). Note the absence of NSC-6 immunolabeling in the cortex (cx) and the striatum (st). (B) NSC-6 immunolabeling of Bergmann glia processes in the molecular layer (mol) of the cerebellum. The granular layer (gr) lacks NSC-6 immunolabeling. (C) NSC-6 labels sparse astrocytes-like cells in the hilus (h), the molecular layer (mol), and the lacunosum moleculare layer (Lmol) of the dentate gyrus. Radial-astrocyte processes—most likely belonging to NSCs due to their perpendicular orientation—in the granule cell layer (gcl) are also immunolabeled. (D) NSC-6 immunolabeling of the SVZ adjacent to the lateral ventricle (lv) and surrounding parenchyma. (E) NSC-6 immunolabeling of the RMS. Note the absence of staining in the striatum (st). (F) NSC-6 immunolabeling of the rostral aspect of RMS, entering and disseminating in the olfactory bulb (ob). The arrow points the rostral direction. (G) Omission of the primary antibody resulted in absence of detectable immunolabeling throughout the entire brain. The corpus callosum between the cortex (cx) and hippocampus (hc) is shown. Scale bars are 50 µm in all images.