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. 2021 Mar 10;11:5546. doi: 10.1038/s41598-021-85129-1

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© The Author(s) 2021, corrected publication 2021

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Immunoblot analysis of NSC-6 immunolabeling in developing mammalian brain. (A) NSC-6 immunoblotting of E15, P1, P30 and P60 mouse whole brain extracts. Four different bands (1–4) and with the respective M_r values of 51 kDa, 47 kDa, 38 kDa, and 35 kDa are recognized by the NSC-6 Ab. These distinct species changed through development and maturation. Immunoblots of the same samples probed with antibodies against GFAP (B), vimentin (C), and Nestin (D). The asterisks mark the bands corresponding to the expected M_r of these proteins. (E) NSC-6 immunoblotting of fetal and adult human brain. Note the distinct pattern of immunolabeling for the four bands a–d, with respective M_r values of 51 kDa, 45 kDa, 38 kDa, and 35 kDa, between fetal and adult brain. (F) NSC-6 immunoblotting of samples from human cortex (cx), hippocampus (hc), cerebellum (cb), brainstem (bs), and spinal cord (sc). Separate blots from different gels were cropped and separated by white space. Entire blots are not available.