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. 2021 Mar 10;11:5589. doi: 10.1038/s41598-021-84984-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Relative leukocyte telomere length (RLTL) dynamics in dairy cattle. All RLTL measurements were pre-adjusted for qPCR plate and qPCR row to account for known sources of measurement error. (a) Age in years at second RLTL measurement is significantly associated with RLTL change. (b) At all measurement times the present measurement (RLTL at time t) is clearly correlated with the previous measurement (RLTL at time t − 1) (estimate = 0.38, P < 0.001). The red line represents a perfect correlation. (c) RLTL over age in years. (d) RLTL over sample year. (e) Longitudinal RLTL change over age for all animals with at least 7 samples as example for RLTL variation. (f) Early-life change in RLTL over age at sampling and (g) over sampling year.