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. 2021 Mar 11;11:5804. doi: 10.1038/s41598-021-83930-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transmission electron microscopy imaging and proteome analysis. (A) Representative electron micrographs of inactivated SARS-CoV-2. The group of virus particles was seen on the grid (Scale bars: 500 nm, 100 nm, 50 nm). (B) Proteome analysis of inactivated SARS-CoV-2 product. (C) Graphs showing real-time cell analysis of gamma-irradiated inactivated SARS-CoV-2 and no—virus control cultured on Vero for 21 days to determine the presence/absence of cytopathic effect in a dose-dependent manner. The Red line is no virus Vero internal control and the Green line is 10 × TCID50 doses of infective SARS-CoV-2 as a positive control.

Transmission electron microscopy imaging and proteome analysis. (A) Representative electron micrographs of inactivated SARS-CoV-2. The group of virus particles was seen on the grid (Scale bars: 500 nm, 100 nm, 50 nm). (B) Proteome analysis of inactivated SARS-CoV-2 product. (C) Graphs showing real-time cell analysis of gamma-irradiated inactivated SARS-CoV-2 and no—virus control cultured on Vero for 21 days to determine the presence/absence of cytopathic effect in a dose-dependent manner. The Red line is no virus Vero internal control and the Green line is 10 × TCID50 doses of infective SARS-CoV-2 as a positive control.