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. 2021 Mar 15;11:5931. doi: 10.1038/s41598-021-85046-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Generation and characterization of C. elegans gst-4 mutant. (a) Diagram of DNA sequences of wild type C. elegans genomic gst-4 and the gst-4(lp11) mutant. Lines represent introns, rectangles represent exons, and dashed line represents the lp11 allele CRISPR deletion. (b) Lifespan of wild type, gst-4(lp10), and gst-4(lp11), statistics in Supplementary Table S1, S2. (c) Lifespan of wild type and gst-4(lp11) mutants consuming either 0% control or 0.4% glucose fed OP50 E. coli, statistics in Supplementary Tables S1, S2.