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. 2021 Mar 17;11:6135. doi: 10.1038/s41598-021-85523-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of PC10:0/10:0, PC20:0/20:0 or solvent on the intracellular ROS level and H₂O₂-induced cytotoxicity. Cells were incubated with the solvent EtOH (0.2%) (set as 100%), PC10:0/10:0 or PC20:0/20:0 (10 µM) in absence (a,b) or presence (c) of 500 µM H₂O₂ for 18 h before intracellular ROS level and H₂O₂-induced changes in cell viability were examined. Intracellular ROS level measured by using CellROX Green in (a) SH-SY5Y cells (n = 13) and (b) Neuro2a cells (n = 12). (c) H₂O₂-induced changes in the viability of SH-SY5Y cells determined by labeling dead cells with propidiumiodide (n = 4). Error bars represent SEM. Asterisks show the statistical significance calculated by one-way ANOVA followed by post hoc testing using Tukey's test (* p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001). Figure was created using Origin Pro 2020b and CorelDRAW Graphics Suite 2020.