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. 2021 Mar 17;11:6135. doi: 10.1038/s41598-021-85523-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of PC10:0/10:0, PC20:0/20:0 or solvent on the intra- and extracellular βHB level. Cells were incubated with the solvent EtOH (0.2%) (set as 100%), PC10:0/10:0 or PC20:0/20:0 (10 µM) for 18 h before the level of βHB was measured by using the β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit. βHB level in the homogenates of (a) SH-SY5Y cells (n = 8) and (b) Neuro2a cells (n ≥ 5). βHB level in the conditioned medium of (c) SH-SY5Y cells (n = 8) and (d) Neuro2a cells (n ≥ 5). Error bars represent SD. The statistical significance was calculated by one-way ANOVA followed by post hoc testing using Tukey's test, no significant differences were observed. Figure was created using Origin Pro 2020b and CorelDRAW Graphics Suite 2020.