Table 1.
Impact of PC10:0/10:0, PC20:0/20:0 or solvent on total cell numbers and cell viability.
| Total cell number (%) | SH-SY5Y | Neuro2a | ||||
|---|---|---|---|---|---|---|
| Solvent | PC10:0/10:0 | PC20:0/20:0 | Solvent | PC10:0/10:0 | PC20:0/20:0 | |
| Mean | 100.0 | 103.4 | 101.6 | 100.0 | 96.9 | 94.7 |
| SEM | 2.2 | 1.8 | 1.8 | 1.9 | 2.2 | 2.9 |
| Number of independent experiments (n) | 53 | 51 | 51 | 24 | 24 | 24 |
| p-value solvent vs. PC10:0/10:0 | 0.424 | 0.623 | ||||
| p-value solvent vs. PC20:0/20:0 | 0.825 | 0.265 | ||||
| p-value PC10:0/10:0 vs. PC20:0/20:0 | 0.789 | 0.799 | ||||
| Dead cells (% of total cells) | SH-SY5Y | Neuro2a | ||||
|---|---|---|---|---|---|---|
| Solvent | PC10:0/10:0 | PC20:0/20:0 | Solvent | PC10:0/10:0 | PC20:0/20:0 | |
| Mean | 2.7 | 2.4 | 3.3 | 7.5 | 8.4 | 7.3 |
| SD | 1.4 | 1.0 | 1.2 | 2.4 | 4.6 | 2.4 |
| Number of independent experiments (n) | 8 | 8 | 7 | 8 | 8 | 7 |
| p-value solvent vs. PC10:0/10:0 | 0.891 | 0.853 | ||||
| p-value solvent vs. PC20:0/20:0 | 0.644 | 0.992 | ||||
| p-value PC10:0/10:0 vs. PC20:0/20:0 | 0.386 | 0.800 | ||||
Total cell number (%): DAPI signal correlating with total cell number of all wells used for the measurements shown in Figs. 1a–e and 2a,b.
Dead cells (%): the percentage of dead cells after treatment with the solvent EtOH (0.2%), PC10:0/10:0 or PC20:0/20:0 (10 µM) for 18 h was calculated after determination of the total cell number and the number of dead cells utilizing DAPI (1 µg/ml) and Ethidium homodimer-1 (10 µM), respectively.
The statistical significance was calculated by one-way ANOVA followed by post hoc testing using Tukey's test, no significant differences were observed.
SEM standard error of the mean, SD standard deviation.