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. 2021 Mar 17;11:6095. doi: 10.1038/s41598-021-85624-5

Figure 1.

Figure 1

Multiple sequence alignment and phylogeny of Bm86 orthologs. (A) Alignment of orthologous Bm86 protein sequences in hard and soft ticks generated in Clustal Omega and BoxShade (https://www.ch.embnet.org/software/BOX_form.html). The letters with a black background are identical amino acids, while gray background letters are similar under the 50% majority rule. Red arrows span the amino acid sequences that were used to generate the recombinant EGF-like domains 1, 2, and 3, as denoted by the red boxes. EGF-1 and EGF-2 are Is86-1 and Is86-2 sequence-specific, respectively, while EGF-3 is sequentially identical for both Is86-1 and Is86-2. (B) Phylogenetic relationship of Bm86 orthologs among hard and soft ticks based on maximum likelihood tree. The numbers by the nodes represent a percent support using 1000 bootstrap replicates. A single asterisk indicates the putative sequences of Is86 recently added to the NCBI GenBank database, two asterisks indicate putative Bm86 orthologs in the soft tick species O. savignyi, and three asterisks denote ATAQ and Bm86-related homologs.