Figure 8:

Material properties of demineralized bone as a function of indentation load (or indentation depth) in a quasistatic multiple load indentation experiment show three different regions with primary contribution towards material property from i.e. surface roughness (with indentation depth varying from 0 to ∼75 nm shown by grey color), collagen fibrils (with indentation depth varying from ∼75 nm to ∼500 nm represented by red) and collagen fibers (with indentation depth varying from larger than ∼500 nm represented by green) (a) Reduced modulus with indentation load (b) Contact hardness with indentation load.