Figure 3.
Dynamics of intracellular calcium concentration during the VP generation in pea seedlings. (a). Dynamics of the fluorescence intensity of a Ca2+-sensitive fluorescent dye fluo-4, AM, loaded into stem cells, induced by local heating of the second adult leaf of pea seedling. The inset shows the fluorescence image of pea seedling stem cells loaded with fluo-4, AM fluorescent probe. (b). Changes of the electrical potential (VS) and intracellular Ca2+ concentration (investigated using the fura-2, AM ratiometric fluorescent dye) in pea stem induced by local heating of the second adult leaf. The black arrow indicates the start of the local heating