Figure 2.

Design of sgRNA for TRE1 edition, located in the substrate binding domain of the trehalase enzyme

(A) Conserved amino acids in blue are the conserved signatures of the substrate binding site. Asterisks are in conserved motifs in this domain. The S→T change present in Selaginella is shown in red. (B) WebLogo of the consensus sequence of extant Viridiplantae accessions; position 13 indicates the overrepresentation of the amino acid Serine in the substrate-binding site, whereas in drought-tolerant plants such as S. moellendoffii, it is replaced by threonine. (http://weblogo.berkeley.edu/). (C) and (D) Position of the sgRNA in Arabidopsis and maize TRE1 genes, respectively. Green boxes correspond to exons, solid black lines, to introns. Red box shows the position of the sgRNA, and PAM is shown in green.