Table 1.

Details of primers and probes used

Target	Forward primer	Reverse primer	Probe sequence and label (chlorophore)	Amplicon size	Annealing temperature	Reference
tet(M)	GTR AYG AAC TTT ACC GAA TC	ATC GYA GAA GCG GRT CAC	N/A	633bp	55°C	[31]
Int and Xis genes	CGC CAAAGG GTC TTG TAT ATG	GCT GTA GGT TTT ATC AGC TTT TGC	N/A	650bp	58°C	[50]
Long A (position 38–9,884 bp)	GGA CTT ATC CAC TTT ATC AAG G	AAA CAG AAG CAG TGA GAA GA	N/A	9806bp	58°C	[51,52]
Long B (Position 9,824–17,947 bp)	GAA AAC TTT AGT GAT TGG TGG	CTG TAG GAA GAT ACT TCA CG	N/A	8123bp	58°C	[53]
Circular intemidate (conventional PCR)	CGT GAA GTA TCT TCC TAC A	AC CTT GAT AAA GTG TGA TAA	N/A	166bp	56°C	[54]
Circular intemidate (ddPCR)	CGT GAA GTA TCT TCC TAC A	GAC CTT GAT AAA GTG TGA TAA	AAT ACT CGA AAG CAC ATA GAA TAA GGC FAM/HEX	167bp	56°C	[41]
Int and Xis (ddPCR)	ATA CTC CCA TAC AGT CAA TAG TCC	AGT TCC ACC CCT GCA TGG	CCG TCG CAGGCA ATG AGT ATG GCT FAM	88bp	56°C	[41]