Figure 6.

Interactions of GST-PINX1-HIS and Pfa1-HIS with Prp43-HIS are mutually exclusive in vitro. (A) Pull-down experiments. GST-PINX1-HIS, Pfa1-HIS and Prp43-HIS (lanes 1 and 2) or GST-PINX1-HIS and Pfa1-HIS (lanes 3 and 4), were mixed prior to pull down of GST-PINX1-HIS and associated proteins with glutathione sepharose. Pellet (IP) and supernatant fractions (Sup) were then processed as described in the legend of Fig. 1. HIS-tagged proteins were detected by Western using HRP-coupled anti-histidine antibodies. (B) As in (A), except that immunoprecipitations were carried out with anti-PINX1 antibodies