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. 2021 Mar 18;16(3):e0245075. doi: 10.1371/journal.pone.0245075

Fig 5. Determination of the biological efficacy of retinoic acid in MKN-45 cells.

Fig 5

After treating MKN cells with 1 μM retinoic acid for 24 hours, SLC2A1 mRNA and GLUT1 protein expression was checked against the control group by (A) RT-PCR and (B) immunoblotting, respectively. GAPDH and β-actin were used as internal controls. (C) Under the same conditions as (A), the nucleus (DAPI) and GLUT1 expression and location were identified by immunocytochemistry. (D) The survival rate of MKN-45 cells was observed after 72 hours of treatment with retinoic acid in MKN-45 cells at concentrations ranging from 10 nM to 100 μM.