Fig 5. Microfibril incorporation of GFP-FBN1 cMFS variants.

FS2 fibroblasts were co-cultured for 5 days with HEK293T cells transiently transfected to express GFP-FBN1 (WT) or variants associated with cMFS. Co-cultures were then fixed and stained with anti-GFP and anti-FBN1 antibodies without permeabilisation [16, 26]. In contrast to the nMFS variants, co-cultures expressing GFP-cMFS variants produced microfibril networks containing readily detectable recombinant material (white arrows). Bar = 100 μm.