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. 2021 Mar 8;17(3):e1009393. doi: 10.1371/journal.ppat.1009393

Fig 5. Key region in ADAM17 responsible for CSFV entry.

Fig 5

(A) Schematic diagram of pADAM17, zfADAM17 and construction of the chimera ADAM17s. Chimera ADAM17s were constructed by replacing the indicated fragments of zfADAM17 with those of pADAM17. SP, signal peptide; PRO, pro-domain; MP, metalloproteinase domain (N223-V477); DD, disintegrin domain; CD, catalytic domain; TM, transmembrane domain; ICD, intracellular domain. (B) Relative binding of sE2-Fc with PK15 (WT), ADAM17-KO and ADAM17-KO stably transduced with pADAM17 (+pADAM17), zfADAM17 (+zfADAM17) or various ADAM17 chimeras by flow cytometry analysis. (C) Above cell lines were infected with indicated pseudoparticles. The lower limit of detection was 10 FFU/ml (dashed line). Asterisks indicate samples below the limit. (D) Western blotting of chimera ADAM17s in the cell lysates using anti-Flag antibody, with anti-GAPDH antibody as the control. Error bars in (B) and (C) indicate SD of the mean (n = 3). The data represent three independent experiments.