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. 2021 Mar 8;17(3):e1009366. doi: 10.1371/journal.ppat.1009366

Fig 5. JMJD6 promotes activated IRF3 ubiquitination.

Fig 5

(A) Endogenous JMJD6 regulates the stability of IRF3. KO-control cells, KO-JMJD6 cells, or KO-JMJD6 cells reconstituted with JMJD6 were infected with SeV for 6 h and then cultured in the presence of cycloheximide (CHX) for 0, 6, 12 h. Total IRF3, Myc-JMJD6, and β-actin were detected. (B) HEK293T cells were transfected with the indicated plasmids and then stimulated with poly(I:C) in the presence or absence of MG132 (20 μM), CQ (100 μM), or Z-VAD-FMK (50 μM) for 6 h. The expression of HA-IRF3 and Myc-JMJD6 proteins were detected by Western blotting. (C) Endogenous JMJD6 enhanced the ubiquitination of IRF3. KO-control cells, KO-JMJD6 cells, or KO-JMJD6 cells reconstituted with JMJD6 were infected with SeV for 6 h in the presence of MG132 (20 μM). Immunoblot analysis (with anti-Ub) of proteins immunoprecipitated (with anti-IRF3) was performed.