10× PfuUltra II Fusion buffer	2.5 μl (1×)
10 mM dNTP mix	0.6 μl (240 μM)
10 μM forward primer	2.5 μl (1 μM)
10 μM reverse primer	2.5 μl (1 μM)
100 ng protein expression plasmid	0.2-5 μl
PfuUltra II Fusion High-Fidelity DNA polymerase	1 μl
Nuclease-free water	Up to 25 μl.