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. 2021 Mar 17;19:112. doi: 10.1186/s12967-021-02709-5

Table 1.

Primer sequences for RT-qPCR

Gene Primer sequences
KPNA2 (human) F: 5′-CTGGGACATCAGAACAAACCAAG-3′
R: 5′-ACACTGAGCCATCACCTGCAAT-3′
CBX8 (human) F: 5′-CCTTCGAAACATGGGTTTGT-3′
R: 5′-CTGGGCTTGTCATCCACTCT-3′
PRDM1 (human) F: 5′-CCCTCATCGGTGAAGTCTA-3′
R: 5′-ACGTAGCGCATCCAGTTG-3′
c-FOS (human) F: 5′-AGAATCCGAAGGGAAAGGAA-3′
R: 5′-CTTCTCCTTCAGCAGGTTGG-3′
U6 F: 5′-CGGCGGTCGTGAAGCGTTCCAT-3′
R: 5′-CCAGTGCAGGGTCCGAGGTAT-3′
GAPDH F: 5′-CACCCACTCCTCCACCTTTG-3′
R: 5′-CCACCACCCTGTTGCTGTAG-3′

RT-qPCR: reverse transcription quantitative polymerase chain reaction; F: forward; R: reverse; KPNA2: karyopherin α2; CBX8: Chromobox 8; PRDM1: PR domain zinc finger protein 1; c-FOS: Cellular oncogene fos; GAPDH: glyceraldehyde-3-phosphate dehydrogenase