Table 1.
Gene | Primer sequences |
---|---|
KPNA2 (human) | F: 5′-CTGGGACATCAGAACAAACCAAG-3′ |
R: 5′-ACACTGAGCCATCACCTGCAAT-3′ | |
CBX8 (human) | F: 5′-CCTTCGAAACATGGGTTTGT-3′ |
R: 5′-CTGGGCTTGTCATCCACTCT-3′ | |
PRDM1 (human) | F: 5′-CCCTCATCGGTGAAGTCTA-3′ |
R: 5′-ACGTAGCGCATCCAGTTG-3′ | |
c-FOS (human) | F: 5′-AGAATCCGAAGGGAAAGGAA-3′ |
R: 5′-CTTCTCCTTCAGCAGGTTGG-3′ | |
U6 | F: 5′-CGGCGGTCGTGAAGCGTTCCAT-3′ |
R: 5′-CCAGTGCAGGGTCCGAGGTAT-3′ | |
GAPDH | F: 5′-CACCCACTCCTCCACCTTTG-3′ |
R: 5′-CCACCACCCTGTTGCTGTAG-3′ |
RT-qPCR: reverse transcription quantitative polymerase chain reaction; F: forward; R: reverse; KPNA2: karyopherin α2; CBX8: Chromobox 8; PRDM1: PR domain zinc finger protein 1; c-FOS: Cellular oncogene fos; GAPDH: glyceraldehyde-3-phosphate dehydrogenase