Table 2.
Strains and plasmids used in this study
| Strains | Description and relevant features | references |
|---|---|---|
| S. Typhimurium ST4/74 | NalR | [30] |
| ST4/74 ΔinvA | Non-polar invA deletion mutant | This study |
| ST4/74 ΔSTM3254 | Non-polar STM3254 deletion mutant with zero growth phenotype in galactitol | [29] |
| ST4/74 ΔSTM3254::KanR | Mutant with allelic exchange of STM3254 against a kanamycin resistance gene | This study |
| ST4/74 ΔgatR-HTH | Partial non-polar deletion of gatR lacking the nucleotides encoding the helix-turn-helix motif (HTH) | This study |
| E. coli | ||
| DH5α | F– endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80ΔlacZΔM15 Δ(lacZYA-argF )U169, hsdR17 (rK–mK+), λ– | |
| Plasmids | ||
| pBR322 | AmpR, TetR | [31] |
| pBR-STM3254 | pBR322 with gene STM3254 | This study |
| pBR-gatR | pBR322 with repressor gene gatR | This study |
| pKD4 | KanR, pir-dependent, FRT sites | [32] |
| pKD46 | λ-Red helper plasmid, AmpR | [32] |
| pCP20 | FLP recombinase plasmid, CmR, AmpR | [32] |