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. 2021 Mar 11;134(6):jcs256578. doi: 10.1242/jcs.256578

Fig. 2.

Fig. 2.

V-type ATPase inhibition progressively increases lysosomal pH. (A) Effects of DMSO [0.1% (v/v)] and bafilomycin A1 (1 µM) on the fluorescence of LysoTracker Red. (B-C) Representative images showing LysoTracker Red fluorescence (B) or fluorescein-dextran ratio (C) of cells treated with bafilomycin A1 (1 µM) or DMSO for the indicated time. Cells were maintained at room temperature or in culture conditions. In the latter, the final hour of incubation was performed at room temperature in HBS. Scale bars: 50 µm. An increase in fluorescein dextran ratio corresponds to an increase in lysosomal pH. (D,E) Summary data (n=1-5) quantifying the effect of bafilomycin A1 on LysoTracker Red fluorescence (D) and fluorescein-dextran ratio (E). The data in D are expressed relative to DMSO.