Fig. 3.

Acute V-type ATPase inhibition potentiates Ca²⁺ signals evoked by GPN and thapsigargin. (A) Effects of acute DMSO [0.1% (v/v)] and bafilomycin A1 (1 µM) treatment on GPN-evoked Ca²⁺ signals. Experiments were performed at room temperature in the absence of external Ca²⁺. (B) Effects of DMSO or a 1-2 h treatment with bafilomycin A1 (1 µM) on GPN-evoked Ca²⁺ signals. (C) Summary data quantifying the effect of bafilomycin A1 on the area under the curve (AUC) of the GPN responses (n=3-5). (D-F) Similar to A-C, except cells were stimulated with thapsigargin (1 µM) in place of GPN (n=3-4). *P<0.05, **P<0.01 (paired-samples t-tests).