Fig. 4.

Sustained V-type ATPase inhibition selectively inhibits GPN-evoked Ca²⁺ signals. (A-D) Effects of DMSO (A), or a 2 h (B), 3 h (C) or 5 h (D) treatment with bafilomycin A1 (1 µM) on GPN-evoked Ca²⁺ signals. Cells were incubated under culture conditions except for the final 1 h when they were loaded with Fura-2 at room temperature in the continued presence of bafilomycin A1. (E) Summary data quantifying the effect of bafilomycin A1 on the magnitude of the GPN responses (n=4-5). (F-J) Similar to A-E, except cells were stimulated with thapsigargin (1 µM) in place of GPN (n=3-5). *P<0.05, **P<0.01 (paired-samples t-tests).