Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 22;10:e63101. doi: 10.7554/eLife.63101

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021, Brovero et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

PMC Copyright notice

Figure 3. — Maximum intensity projections of brain and ventral nerve cord tissues from 4- to 7-days old male and female flies. The fru P1 ∩ DIP intersecting neurons are labeled with green (rabbit α-GFP Alexa Flour 488), and neuropil are labeled with magenta (mouse α-nc82, Alexa Flour 633). The genotype is DIP-Gal4/10xUAS > stop > GFP.Myr; fru P1^FLP, except for DIP-ι. This Gal4 transgenic strains was generated using a CRISPR-mediated insertion of the T2A-Gal4 with the dominant 3xP3-GFP marker. For this strain, 10xUAS > stop > myr::smGdP-cMyc was used and fru P1 ∩ DIP intersecting neurons are labeled with red (rabbit α-Myc, Alexa Flour 568) and then false-colored to green. The neuropil are labeled with magenta (mouse α-nc82, Alexa Flour 633). One Gal4 did not show consistent expression upon intersecting: DIP-iota. DIP-iota was tested with 10xUAS-RFP, and showed expression outside of fru P1 neurons.

Figure 3—figure supplement 1. — Maximum intensity projections of brain and ventral nerve cord tissues from 4- to 7-days old male and female flies. The fru P1 ∩ DIP intersecting neurons are labeled with green (rabbit α-GFP Alexa Flour 488), and neuropil are labeled with magenta (mouse α-nc82, Alexa Flour 633). The genotype is DIP-Gal4/10xUAS > stop > GFP.Myr; fru P1^FLP, except for DIP-ι. This Gal4 transgenic strains was generated using a CRISPR-mediated insertion of the T2A-Gal4 with the dominant 3xP3-GFP marker. For this strain, 10xUAS > stop > myr::smGdP-cMyc was used and fru P1 ∩ DIP intersecting neurons are labeled with red (rabbit α-Myc, Alexa Flour 568) and then false-colored to green. The neuropil are labeled with magenta (mouse α-nc82, Alexa Flour 633). One Gal4 did not show consistent expression upon intersecting: DIP-iota. DIP-iota was tested with 10xUAS-RFP, and showed expression outside of fru P1 neurons.