REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
2-43	Liu et al., 2020b	N/A
2-15	Liu et al., 2020b	N/A
2-4	Liu et al., 2020b	N/A
H4	Wu et al., 2020b	N/A
anti-VSV-G antibody	ATCC	Cat#CRL-2700; RRID:CVCL_G654
Bacteria and virus strains
SARS-CoV-2 pseudovirus	Liu et al., 2020b	N/A
Chemicals, peptides, and recombinant proteins
polyethylenimine	Polysciences, Inc.	Cat# 23966
Turbo293	Speed BioSystems	Cat#PXX1002
HRV-3C protease	MilliporeSigma	Cat# SAE0045
SARS-CoV-2 spike S2P	Wrapp et al., 2020	N/A
SARS-CoV-2 spike	provided by Dr. Peihui Wang, China	N/A
SARS-CoV-2 RBD	This study	N/A
Critical commercial assays
Strep-Tactin XT Superflow 50%	Zymo research	Cat#P2004-1-5
Sensor Chip CM5	Cytiva	Cat#BR100030
His Capture Kit	Cytiva	Cat#28995056
Glycine 1.5	Cytiva	Cat# BR100354
HBS-EP+ Buffer	Cytiva	Cat# BR100826
QuikChange II site directed mutagenesis kit	Agilent	Cat# 200524
rProtein A Sepharose	GE	Cat#17-1279-01
NI-NTA	GE Health care	Cat# 17-0921-09
Superdex 200 Increase 10/300 GL	Cytiva	Cat# 28990945
Pierce Fab Preparation Kit	Thermo fisher	Cat# 44985
Deposited data
New IGVL2-14 gene allele (IGVL2-14∗0X)	This study	ENA: PRJEB31020
Cryo-EM structures: SARS-CoV-2 spike	Walls et al., 2020	PDB ID: 6VXX
Cryo-EM structures: SARS-CoV-2 spike	Henderson et al., 2020	PDB ID: 6X2C
Cryo-EM structures: SARS-CoV-2 spike	Herrera et al., 2020	PDB ID: 6X6P
Cryo-EM structures: SARS-CoV-2 spike	Henderson et al., 2020	PDB ID: 6X29
Cryo-EM structures: SARS-CoV-2 spike	McCallum et al., 2020	PDB ID: 6X79
Cryo-EM structures: SARS-CoV-2 spike	Zhou et al., 2020	PDB ID: 6XLU
Cryo-EM structures: SARS-CoV-2 spike	Zhou et al., 2020	PDB ID: 6XM5
Cryo-EM structures: SARS-CoV-2 spike	Cai et al., 2020	PDB ID: 6XR8
Cryo-EM structures: SARS-CoV-2 spike	Toelzer et al., 2020	PDB ID: 6ZB4
Cryo-EM structures: SARS-CoV-2 spike	Wrobel et al., 2020	PDB ID: 6ZGE
Cryo-EM structures: SARS-CoV-2 spike	Wrobel et al., 2020	PDB ID: 6ZGI
Cryo-EM structures: SARS-CoV-2 spike	Xiong et al., 2020	PDB ID: 6ZOX
Cryo-EM structures: SARS-CoV-2 spike	Xiong et al., 2020	PDB ID: 6ZOY
Cryo-EM structures: SARS-CoV-2 spike	Xiong et al., 2020	PDB ID: 6ZOZ
Cryo-EM structures: SARS-CoV-2 spike	Xiong et al., 2020	PDB ID: 6ZP0
Cryo-EM structures: SARS-CoV-2 spike	Xiong et al., 2020	PDB ID: 6ZP1
Cryo-EM structures: SARS-CoV-2 spike	Xiong et al., 2020	PDB ID: 6ZP2
Cryo-EM structures: SARS-CoV-2 spike	Bangaru et al., 2020	PDB ID: 7JJI
Cryo-EM structures: SARS-CoV-2 spike	Wrobel et al., 2020	PDB ID: 6ZGG
Crystal structure: SARS-CoV-2 RBD	Wu et al., 2020b	PDB ID: 7BZ5
Crystal structure: CH65 UCA	Schmidt et al., 2013	PDB ID: 4HK0
Cryo-EM structure: 2-4	Liu et al., 2020b	PDB ID: 6XEY
Cryo-EM structure: S2M11	Tortorici et al., 2020	PDB ID:7K43
Cryo-EM structure: C121	Barnes et al., 2020a	PDB ID: 7K8X
Cryo-EM structure: C002	Barnes et al., 2020a	PDB ID: 7K8T
Cryo-EM structure: C144	Barnes et al., 2020a	PDB ID: 7K90
Cryo-EM structure: BD23	Cao et al., 2020	PDB ID: 7BYR
Cryo-EM structure: B38	Wu et al., 2020b	PDB ID: 7BZ5
Cryo-EM structure: S2H13	Tortorici et al., 2020	PDB ID: 7JV2
Cryo-EM structure: S309	Pinto et al., 2020a	PDB ID: 6WPS
Cryo-EM structure: C119	Barnes et al., 2020a	PDB ID: 7K8W
Cryo-EM structure: C102	Barnes et al., 2020a	PDB ID: 7K8M
Cryo-EM structure: C104	Barnes et al., 2020a	PDB ID: 7K8U
Cryo-EM structure: C110	Barnes et al., 2020a	PDB ID: 7K8V
Cryo-EM structure: S2E12	Tortorici et al., 2020	PDB ID:7K45
Cryo-EM structure: C135	Barnes et al., 2020a	PDB ID: 7K8Z
Cryo-EM structure: COVA1-16	Liu et al., 2020a	PDB ID: 7JMW
Cryo-EM structure: P17	Yao et al., 2021	PDB ID: 7CWO
Crystal structure: BD-236	Du et al., 2020	PDB ID: 7CHB
Crystal structure: BD-368-2	Du et al., 2020	PDB ID: 7CHE
Crystal structure: BD-629	Du et al., 2020	PDB ID: 7CH5
Crystal structure: P2C-1F11	Wang et al., 2020	PDB ID: 7CDI
Crystal structure: P2D-1A3	Wang et al., 2020	PDB ID: 7CDJ
Crystal structure: EY6A	Zhou et al., 2020	PDB ID: 6ZER
Crystal structure: CV07-250	Kreye et al., 2020	PDB ID: 6XKQ
Crystal structure: CV07-270	Kreye et al., 2020	PDB ID: 6XKP
Cryo-EM structure: S309	Pinto et al., 2020b	PDB ID: 6WPT
Crystal structure: P2B-2F6	Ju et al., 2020	PDB ID: 7BWJ
Crystal structure: CB6	Shi et al., 2020	PDB ID: 7C01
Cryo-EM structure: BD-23	Cao et al., 2020	PDB ID: 7BYR
Crystal structure: B38	Wu et al., 2020b	PDB ID: 7BZ5
Crystal structure: COVA2-39	Wu et al., 2020a	PDB ID: 7JMP
Crystal structure: COVA2-04	Wu et al., 2020a	PDB ID: 7JMO
Crystal structure: H014	Lv et al., 2020	PDB ID: 7CAH
Crystal structure: CR3022	Huo et al., 2020	PDB ID: 6YM0
Crystal structure: CV30	Hurlburt et al., 2020	PDB ID: 6XE1
Cryo-EM structure: REGN10933	Hansen et al., 2020	PDB ID: 6XDG
Cryo-EM structure: REGN10987	Hansen et al., 2020	PDB ID: 6XDG
Cryo-EM structure: C105	Barnes et al., 2020b	PDB ID: 6XCN
Cryo-EM structure: CC12.1	Yuan et al., 2020	PDB ID: 6XC2
Cryo-EM structure: CC12.3	Yuan et al., 2020	PDB ID: 6XC4
Crystal structure: 2-15	This study	PDB ID: 7L5B
Cryo-EM structure: 2-43	This study	PDB ID: 7L56; EMDB-23165
Cryo-EM structure: 2-15	This study	PDB ID: 7L57; EMDB-23166
Cryo-EM structure: H4	This study	PDB ID: 7L58; EMDB-23167
Experimental models: Cell lines
Expi293	ThermoFisher	Cat#A14527; RRID:CVCL_D615
HEK293T/17	ATCC	Cat# CRL-11268; RRID:CVCL_1926
Vero E6	ATCC	Cat# CRL-1586; RRID:CVCL_0574
Expi293F GnTI- Cells	Thermo Fisher	Cat# A39240
Recombinant DNA
pVRC8400 vector	https://www.addgene.org	Cat#63160; RRID:Addgene_63164
gWiz	Aldeveron	Cat# 5008
pcDNA3.4	ThermoFisher	Cat# A14697
Software and algorithms
GraphPad Prism 7.01 Software	GraphPad Prism Software, Inc.	N/A
The PyMol Molecular Graphics System, v1.8.6	Schrödinger, LLC	https://pymol.org/2/
IMGT/V-Quest	IMGT	http://www.imgt.org/IMGT_vquest/vquest
SONAR	Schramm et al., 2016	https://github.com/scharch/SONAR
Muscle	Edgar, 2004	https://drive5.com/usearch/
CLUSTALO	Sievers and Higgins, 2018	http://clustal.org/omega/
Python v3.8.3	https://www.python.org/downloads/	N/A
cryoSPARC, v2.15.0	Punjani et al., 2017	https://cryosparc.com/
UCSF Chimera, v1.14	Pettersen et al., 2004	https://www.cgl.ucsf.edu/chimera/
UCSF ChimeraX, v1.1	Goddard et al., 2018	https://www.cgl.ucsf.edu/chimerax/
Schrödinger Release 2020-2:BioLuminate	Zhu et al., 2014	https://www.schrodinger.com/products/bioluminate
ISOLDE, v.1.0.1	Croll, 2018	https://isolde.cimr.cam.ac.uk/
COOT, 0.9	Emsley et al., 2010	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Phenix, v1.18	Adams et al., 2010	https://www.phenix-online.org/;
Leginon, v.3.5beta	Suloway et al., 2005	N/A
XDS	Kabsch, 2010	https://xds.mpimf-heidelberg.mpg.de/
CCP4i (Aimless)	Winn et al., 2011	https://www.ccp4.ac.uk
PDB-redo	Joosten et al., 2014	https://pdb-redo.eu/
MolProbity	Chen et al., 2010	http://molprobity.biochem.duke.edu
Other
Free style Media	Invitrogen	Cat # 12338026