Fig. 5.

The immune activation function after pseudovirus challenge in WT mice and hACE2 mice models. (a) Experimental flowchart in C57BL/6 mice. (b-d) Secretion levels of TNF-α (b), IFN-γ (c) and IFN-β (d) in BALF of WT mice and hACE2 mice. (e) Representative flow cytometric analysis images of F4/80⁺CD80⁺ AMs in BALF of WT mice and hACE2 mice. (f) Relative quantification of F4/80⁺CD80⁺ AMs in BALF of WT mice and hACE2 mice. (g) Relative quantification of CD86⁺CD80⁺ DCs in MLNs of WT mice and hACE2 mice. (h) Relative quantification of CD11c⁺CD103⁺ DCs in MLNs of WT mice and hACE2 mice. (I: PBS, II: Poly(I:C), III: PL-Poly(I:C), IV: NL-Poly(I:C), V: PL-Poly(I:C)/RBD, VI: NL-Poly(I:C)/RBD).