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. 2021 Mar 9;2021:5590855. doi: 10.1155/2021/5590855

Figure 2.

Figure 2

ox-LDL increased the oxidative stress and mitochondrial injury via modulating NRF2. (a) The ROS level of the PBS- or ox-LDL-treated macrophages was determined by immunofluorescent staining. (b) The ROS-positive rates of the PBS- or ox-LDL-treated macrophages with different treatments were determined by flow cytometry. (c) The protein level of NRF2 in the nucleus of the PBS- or ox-LDL-treated macrophages was assessed by Western blot assay. (d) The protein level of NRF2 and KEAP1 in the cytoplasm of the PBS- or ox-LDL-treated macrophages was assessed by Western blot assay. (e) The mitochondrial injury of the PBS- or ox-LDL-treated (6 h) macrophages was assessed by MitoTracker staining. (f) The protein level of DRP1 and FIS1 in the PBS- or ox-LDL-treated (6 h) macrophages with respective treatments was assessed by Western blot assay. N = 3; significant differences between treatment groups were shown as ∗P < 0.05.