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. 2021 Mar 11;2021:6688708. doi: 10.1155/2021/6688708

Figure 1.

Figure 1

Inhibitory effect of FUC on Nrf2-Keap1 binding. (a) The effect of FUC on the conformation of Keap1/Nrf2 complex by IP assay. PC12 cells were pretreated with different concentrations of FUC for 2 h and then exposed to 250 μM 6-OHDA for 24 h. Total protein was extracted and incubated with beads and anti-Keap1 antibody overnight at 4°C. The immunoprecipitated proteins were subjected to SDS-PAGE and detected using anti-Nrf2 antibody (IP), and the total Keap1 protein was detected by anti-Keap1 (IB). ∗P < 0.05 and ∗∗P < 0.01 versus the control group. (b) Kinetic binding analysis of FUC and Keap1 protein. (c) Kinetic binding analysis of Nrf2 protein and Keap1 protein. (d) Kinetic binding analysis of FUC and Nrf2 protein. (e) FUC interfered with Nrf2-Keap1 binding.