Skip to main content
. 2021 Mar 11;2021:6688708. doi: 10.1155/2021/6688708

Figure 5.

Figure 5

FUC prevents 6-OHDA-induced damage of PC12 cells. (a) The effect of FUC on 6-OHDA-induced cellular ROS level in PC12 cells. The PC12 cells were treated with DCFH-DA (10 μM), and the ROS level was detected by a flow cytometer with an excitation wavelength of 488 nm and an emission wavelength of 525 nm. (b) The effect of FUC on 6-OHDA-induced apoptosis. The treated cells were incubated with Annexin V-FITC and PI and then detected by a flow cytometer. The excitation and emission wavelengths of FITC were 488 nm and 530 nm, respectively. The excitation and emission wavelengths of PI were 488 nm and 630 nm, respectively. (c) The effect of FUC on mitochondrial membrane in 6-OHDA-exposed PC12 cells. PC12 cells were pretreated with FUC for 2 h and then exposed to 6-OHDA for 24 h. Subsequently, the cells were then treated with JC-1 probe and imaged by a confocal microscope. ∗∗P < 0.01 versus the control group. #P < 0.05 and ##P < 0.01 versus the 6-OHDA alone treatment group.