Table 1. Oligonucleotide primers used to construct DNA fragments of the canine nicotinamide adenine dinucleotide-cytochrome b5 reductase soluble forms.

Primer	Sequence
F1	5′-GGATCCATGAAGCTGTTCCAG-3′
R1	5′-GAATTCTCAGAAGGCGAAGCAG-3′
F2	5′-GATCCGTGCCCTCATCAAAG-3′
R2	5′-CTTTGATGAGGGCACGGATC-3′

The F1 and R1 primers included BamHI (italic) and EcoRI (underlined) restriction sites, respectively. The F2 and R2 primers included the mutation site in bold.