Table 2. The kinetic properties of the canine nicotinamide adenine dinucleotide-cytochrome b5 reductase soluble forms.
| Wild-type | Mutant | P value | |||
|---|---|---|---|---|---|
| Km [NADH] | µM | 13.8 ± 2.4 | 11.2 ± 6.5 | 0.497 | |
| Kcat | S-1 | 455.8 ± 143.1 | 466.8 ± 161.9 | 0.922 | |
| Kcat/Km | S-1M-1 | 3.4 ± 1.2 × 107 | 4.7 ± 1.7 × 107 | 0.277 | |
| Km [ferricyanide] | µM | 14.5 ± 8.8 | 21.3 ± 2.6 | 0.271 | |
| Kcat | S-1 | 279.9 ± 52.7 | 369.8 ± 56.7 | 0.114 | |
| Kcat/Km | S-1M-1 | 2.3 ± 0.8 × 107 | 1.8 ± 0.4 × 107 | 0.413 | |
The measurement of the nicotinamide adenine dinucleotide (NADH)-ferricyanide activity were performed in triplicates. Data are represented as mean ± standard deviation. The significance of the differences was evaluated using the Student’s t-test.