Figure 6.

CD46-CYT2 regulates human bladder cancer cell migration via hnRNPA1

(A) (1) Representative images of cell culture plates following staining for colony formation of EJ-1 cells expressing FLAG-hnRNPA1 or control plasmid psi-FLAG. (2) Number of colonies was quantified. (B) Migration assay for bladder cancer cells. The number of migrated cells was quantified in five random images from each treatment group. The data represent mean ± SD and were analyzed by an unpaired two-tailed Student’s t test (n = 5). ∗∗∗p < 0.001 versus control. (C and D) EJ-1 cells were infected with different combinations of lentivirus encoding sh-LacZ, sh-CD46-CYT2, psi-FLAG, and/or FLAG-hnRNPA1 as indicated. After 72 h of culture, a colony formation assay and transwell migration assay were performed. ∗∗∗p < 0.001 versus control.