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. 2020 Dec 19;20:569–582. doi: 10.1016/j.omto.2020.12.006

Figure 1.

Figure 1

VB inhibits proliferation, migration, and invasion but promotes apoptosis of GBM cells

(A) The proliferation of U87 and U251 cell lines treated with VB at different concentrations (0, 20, 40, 60, 80, and 100 μΜ) at 0, 24, 48, and 72 h was detected by MTT assay; U87 and U251 cell lines were treated with VB at different concentrations (0, 25, 50, and 75 μΜ). (B) The apoptosis of U87 and U251 cell lines was measured by flow cytometry. (C) The cell migration of U87 and U251 cell lines was measured by Transwell assay. (D) The cell invasion of U87 and U251 cell lines was measured by Transwell assay. (E) The mRNA expression of PCNA, Bcl-2, E-cadherin, MMP9, and N-cadherin was detected by qRT-PCR in U87 and U251 cell lines. (F) The protein expression of PCNA, Bcl-2, E-cadherin, MMP9, and N-cadherin was detected by western blot analysis in U87 and U251 cell lines. Data were shown as mean ± standard deviation of three technical replicates. ANOVA of repeated measurement was used for data comparison in (A) and one-way ANOVA for data comparison in (B−E) with Tukey’s post hoc test. ∗p < 0.05, compared with the previous drug concentration at the same time point; #p < 0.05, compared with the previous time point at the same drug concentration.