Figure 1.

FOXO6 expression was elevated in EC

(A) The expression of FOXO6 in normal esophageal tissues (green) and EC tissues (red) was analyzed in the GEO: GSE26886 dataset. (B) The expression of FOXO6 in normal esophageal tissues (gray) and EC tissues (red) was analyzed in TCGA-ESCA from GEPIA. (C) FOXO6 expression between 38 EC specimens and paired non-cancerous specimens by IHC. (D) FOXO6 expression between 38 EC specimens and paired non-cancerous specimens by qRT-PCR. (E) FOXO6 expression between 38 EC specimens and paired non-cancerous specimens by western blot analysis. (F) FOXO6 protein expression was quantified using ImageJ software normalized to β-actin. (G) FOXO6 protein expression normalized to β-actin in EC cell lines (Kyse30, Kyse140, Kyse450, Kyse510, and Kyse520) and esophageal epithelial cell lines (HEEpiC and HESMC) with western blot analysis. A, adjacent non-cancerous tissues; T, tumor tissues. The levels being reported were relative to the mean expression of three non-cancerous samples (three adjacent non-tumorous esophageal tissues or normal esophageal cells) for each experiment. The data are presented as mean ± standard deviation. The data for tumor and adjacent tissues were analyzed by a Student’s t test; comparison of data of multiple groups was performed by one-way ANOVA. ∗p < 0.05 versus group A or HEEpiC cells.