Figure 3.

Silencing of FOXO6 physically reduced USP7, resulting in promoted apoptosis

(A) The correlation between FOXO6 and USP7 expression was analyzed in TCGA-ESCE database. (B) IHC was used to detect the expression level of USP7 EC tissues and paired non-cancer tissues. (C) The expression level of USP7 was detected by qRT-PCR in FOXO6 knockdown EC cells. (D) Western blot analysis of protein levels of USP7 and c-CASP3 (a splint of CASP3) normalized to β-actin in FOXO6-knockdown EC cells. (E) Flow cytometry analysis was performed to detect the effects of FOXO6 knockdown, USP7 knockdown, or knockdown of both on apoptosis of EC cells. (F) The enrichment of FOXO6 in the USP7 promoter region was detected by ChIP-qPCR. Measurement data are expressed as mean ± standard deviation and compared by a one-way ANOVA among multiple groups. The experiment was repeated three times independently. ∗p < 0.05 versus the si-NC group.