Figure 6.

CLU regulated by JMJD3 correlates with demethylation of H3K27me3

(A) The effect of JMJD3 knockdown on CLU protein levels normalized to β-actin in EC cells was detected by western blot analysis. (B) The effect of JMJD3 knockdown on CLU mRNA expression in EC cells was detected by qRT-PCR. (C) Western blot analysis of the effect of JMJD3 knockdown on JMJD3 protein levels normalized to β-actin in EC cells. (D) In WT and JMJD3 knockout (KO) EC cells, Vec, full-length JMJD3 (JMJD3-fl), and JMJD3-mut plasmids were transferred, respectively, and the expression of proteins was detected by western blot analysis. (E) The enrichment of H3K27me3 in the CLU promoter region was detected by ChIP-qPCR. Measurement data are expressed as mean ± standard deviation and compared by one-way ANOVA among multiple groups. The experiment was repeated three times independently. ∗p < 0.05 versus the si-NC group or WT + Vec group; ^#p < 0.05 versus the WT + JMJD3-mut group or KO + Vec group.