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. 2021 Mar 8;2(1):100382. doi: 10.1016/j.xpro.2021.100382

Growth medium

Reagent Final concentration Amount
Neurobasal medium n/a 48 mL
Glutamax 1% (v/v) 0.5 mL
B27 2% (v/v) 1 mL
Penicillin/streptomycin 1% (v/v) 0.5 mL
Total n/a 50 mL

Keep at 4°C for up to 1 week.